How Clean Is Your BSA?

How Clean Is Your BSA?

Bovine serum albumin (BSA) is a crucial component in many biopharma and biotechnology applications. The primary characteristics –structure, size, purity, and protein content –are the same across different manufacturers and grades of BSA.  However, trace contaminants such as endotoxins, proteases, metals and immunoglobulins vary depending on lot, grade and manufacturer of BSA.  Lot-to-lot differences in contaminants can cause variable performance in final applications such as diagnostics, vaccine manufacturing, and mammalian cell growth for biopharmaceutical manufacturing1,2.  For this reason, it is recommended to assess lot-to-lot contaminant variability when evaluating BSA in a final application. The purpose of this study is to evaluate the consistency of BSA across multiple manufacturers as measured by residual endotoxin quantification and its effect on mammalian cell phenotype using expression of the inflammatory cytokine IL-8 in THP-1 cells.

To view the entire study, please click here.